TABLE 2.
Strains and plasmids used in this study
| Strain/plasmid | Characteristicsa | Source |
|---|---|---|
| Acinetobacter venetianus strains | ||
| WT | Acinetobacter venetianus RAG-1, WT strain | Presented by Professor D L Gutnick |
| ΔF959_RS02655 | F959_RS02655 deletion mutant | This study |
| ΔF959_RS06225 | F959_RS06225 deletion mutant | This study |
| ΔF959_RS06795 | F959_RS06795 deletion mutant | This study |
| ΔaltL | F959_RS14525 (altL) deletion mutant | This study |
| ΔaltL/PaltL | ΔaltL complemented strain | This study |
| ΔalkRa | alkRa deletion mutant | This study |
| ΔalkRa/PalkRa | ΔalkRa complemented strain | This study |
| ΔalmA/alkMa::gfp | Mutant with almA deletion and replacement of alkMa by gfp | This study |
| ΔaltL/almA/alkMa::gfp | Mutant with almA and altL deletion and replacement of alkMa by gfp | This study |
| ΔalmA/alkMa::gfp-pKRG | Mutant ΔalmA/alkMa::gfp containing pKRG plasmid | This study |
| ΔaltL/almA/alkMa::gfp-pKRG | Mutant ΔaltL/almA/alkMa::gfp-pKRG containing pKRG plasmid | This study |
| ΔfadLRa | fadLRa insertion inactivation mutant | This study |
| ΔaltL/fadLRa | altL deletion and fadLRa insertion inactivation mutant | This study |
| Pseudomonas aeruginosa strains | ||
| PAO1 | WT strain | Lab collection |
| ΔaltLPa | PA1764 deletion mutant | This study |
| ΔaltLPa/PaltLPa | ΔaltLPa complemented strain | This study |
| PAO1/P | PAO1 containing pMMB67EH plasmid | This study |
| PAO1/PaltL | PAO1 containing PaltL plasmid | This study |
| Escherichia coli strains | ||
| DH5α | Cloning strain | Lab collection |
| S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | Lab collection |
| Plasmids | ||
| pK18mobsacB | Suicide plasmid for gene knockout, Kanr | Lab collection |
| pKU | Suicide plasmid for gene knockout, Kanr | Lab collection |
| pMMB67EH | Expression vector, Ampr | Lab collection |
| pK18-F959_RS02655-UD | pK18mobsacB containing the 904-bp upstream fragment and 871-bp downstream fragment of F959_RS02655 gene, Kanr | This study |
| pK18-F959_RS06225-UD | pK18mobsacB containing the 787-bp upstream fragment and 728-bp downstream fragment of F959_RS06225 gene, Kanr | This study |
| pK18-F959_RS06795-UD | pK18mobsacB containing the 796-bp upstream fragment and 709-bp downstream fragment of F959_RS06795 gene, Kanr | This study |
| pK18-altL-UD | pK18mobsacB containing the 697-bp upstream fragment and 743-bp downstream fragment of altL gene, Kanr | This study |
| pK18-almA-UD | pK18mobsacB containing the 793-bp upstream fragment and 719-bp downstream fragment of almA gene, Kanr | This study |
| pK18-alkMa-UD | pK18mobsacB containing the 830-bp upstream fragment and 635-bp downstream fragment of alkMa gene, Kanr | This study |
| pK18-gfp-UD | pK18mobsacB containing the 830-bp upstream fragment of alkMa, gfp gene, and 635-bp downstream fragment of alkMa, Kanr | This study |
| pMK | pMMB67EH with replacement of Ampr to Kanr and deletion of lacI | This study |
| pKRG | pMK carrying the alkRa-gfp sequence | This study |
| PaltL | pMMB67EH carrying the altL ORF | This study |
| PalkRa | pMMB67EH carrying the alkRa ORF | This study |
| PaltLPa | pMMB67EH carrying the altLPa ORF | This study |
a
ORF, open reading frame.