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. 2022 Sep 29;12(10):1322. doi: 10.3390/brainsci12101322

Figure 7.

Figure 7

HIF-1α is required in M2 macrophage exosomes to promote VEGF expression and angiogenesis on bEnd.3 cells. qRT-PCR was conducted for analysis of the inhibitory efficiency of HIF-1α-siRNAs (#1, #2, and #3) (A). The protein levels of VEGF in HUVECs treated with M2 macrophage exosomes (B). The mRNA level of VEGF in bEnd.3 cells pretreated with M2 macrophage exosomes (C). The bEnd.3 cell proliferation test (D). Quantification of EdU+ cells in each group (E). Tube formations were assessed after growing bEnd.3 cells pretreated with exosomes or PBS. Scale bar = 500 µm (F–I). Migrated areas were measured in each group on bEnd.3 cells (I). Quantification of the numbers of the migrated bEnd.3 cells (J). Mean ± SD. ## p < 0.01 HIF-1α-siRNA group versus Con siRNA group. N = 5 per group.