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. 2022 Oct 5;12(10):1427. doi: 10.3390/biom12101427

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The self-build hook-delivery device (HDD). (A) The HDD consists of an ALZET^® rat jugular catheter and a small microneedle (Nordson Optimum^®) attached to an osmotic pump (ALZET^® 2006). (B) Close-up view of the catheter’s tip. The maximum length of the microneedle after bending was 8 mm and for the shank inserted in the scala tympani, it was 1.5 mm. (C) Incubation of the pump with attached catheter in a 6-well plate prior to implantation. For quality control, the induced pumping was checked visually by inspection of fluid filling of Eppendorf^® tubes.