Figure 3.

Beta-cell-specific gene delivery of Manf prevented islets from severe insulitis. Light microscope pictures of islets representing no insulitis (A), insulitis grade 1 (C), insulitis grade 2 (E), insulitis grade 3 (G), and insulitis grade 4 (I), are demonstrated as well as graphs of each insulitis grading, comparing the mean percentages of islets in each group (B,D,F,H,J). (K) Total insulitis. Results are presented in means ± SEM (n = 14–17/group). Kruskal-Wallis test, followed by the Dunn’s test was performed for multiple comparisons for finding the significant differences. *, ** and *** denote p < 0.05, p < 0.01 and p < 0.001, respectively.