Figure 4.

Beta-cell-specific gene delivery of Manf increased the content of insulin in islets of NOD mice. Light microscope pictures of NOD mice tissue sections immunohistochemically stained for insulin (A); a representative image of islets of AAV8.ins-Manf treated mice and (C); a representative image of islets of AAV8.ins-GFP treated mice) and graphs over the mean percentage of insulin-positive or insulin-negative islets in each group (B,D). (A) Two microscope pictures representing insulin-positive islets, where brown colour indicates insulin. (B) The percentages of insulin-positive islets in treated mice. (C) Two microscope pictures representing insulin-negative islets. (D) The percentages of insulin-negative islets in treated mice. (E) Percentages of insulin-positive and insulin-negative cells in total. Results are presented in means ± SEM (n = 14–17/group). Kruskal–Wallis test followed by Dunn’s test was performed for multiple comparisons. ** denote p < 0.01.