FIG 2.

The 5-fold replicated synthetic CF microbiomes converge toward a single stable state in the absence of antibiotic perturbations. Five replicate synthetic microbiomes were grown anaerobically in artificial sputum medium. The community composition was estimated by 16S rRNA gene amplicon sequencing at time 0 and at every 2-day passage (x axes) into fresh medium (10% transfer of 2 mL culture volume). The colored bars represent the relative abundance of each species in the community (left y axis), while the black line represents the total bacterial abundance per mL (right y axis, log scale). Each panel represents a separate replicate experiment. Strain information is provided in Table 1 (our default P. aeruginosa strain is mucoid PDO300).