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. 2022 Sep 25;12(10):1293. doi: 10.3390/brainsci12101293

Figure 5.

Figure 5

Endothelial cells grow in the direction of the laminar flow in dynamic BBB model. BBB was cultured under static conditions (A) or under a laminar flow of 4–15 dyn/cm2 (B) for 8 days Confocal images obtained following staining of the hCMEC/D3 cells for DAPI (blue), and ZO-1 (green) merged when grown on collagen-coated microporous membranes. Arrows indicate the direction of the flow. Images were taken using an UltraVIEW confocal microscope.