Table 4.

List of publications questioning the diagnostic value of EVs in AITDs. HC—Healthy Controls; HT—Hashimoto’s Thyroiditis; GD—Graves’ Disease; (d) UC—(differential) UltraCentrifugation; WB—Western Blot; qRT-PCR—quantitative Real-Time Polymerase Chain Reaction; DEcRs—Differentially Expressed circRNAs.

Reference	Initial Material	Patients	EV Isolation Technique	Content Analysis	Findings
(Cui et al., 2019) [73]	Serum	30 HT, 30 HC	High-speed dUC	WB	TPO, HSP60, MHCII: HT > HC. No difference in TG, HMGB1, ICAM1
(Cui et al., 2021) [75]	Serum	33 GD, HC	High-speed dUC	WB	IGF-1R, HSP60: GD > HC
(Jia et al., 2021) [80]	Plasma	12 GD, 10 HT, 7 HC	High-speed dUC	Proteomics	List of differential proteins implicated in the immune and metabolic systems
(Rodríguez-Muñoz et al.) [76]	Plasma	33 GD, 29 HT, 45 HC	Medium-speed UC	qRT-PCR	Mir-146a, miR-155: AITD > HC
(Sun et al., 2020) [82]	Plasma	25 GD, 25 HC	High-speed dUC	CircRNA microarray	15 DEcRs hsa_circRNA_000102: GD > HC