Figure 3.

TH5478 increases 8-oxoG content but decreases DNA strand breakage. (A) Immunofluorescence staining of pancreas tissue sections for 8-oxoG (red) as the index of oxidative DNA damage. Nuclei were counterstained with DAPI (blue). Magnification was 400×. The scale bar is 50 µm. (B) The intensity of 8-oxoG increased significantly in the TH5487-treated group compared to the cerulein group. (C) The level of DNA strand breakage in primary acinar cells was detected by alkaline comet assay. Prior to the 100 nM cerulein treatment, cells were preincubated with 5 µM TH5487 for 1 h. (D) Cerulein treatment induced DNA breakage and OGG1 inhibition reduced the amount of DNA breakage. Values are presented as mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. cerulein group*.