Figure 2.

PHGDH inhibition affects mitochondrial cellular respiration under normoxic conditions. (A) Representative experiment showing the oxygen consumption rates (OCR) of normoxic HCT116 (top) and DLD-1 (bottom) was measured upon treatment with NCT-503 (16 h) at indicated concentrations after consecutive injection at indicated time points of oligomycin, FCCP, rotenone and antimycin A using the Seahorse analyzer. The OCR was expressed as pmoles/min/µg protein. (B) Summary graphs showing basal respiration; (C) summary graphs showing ATP production; (D) summary graphs showing the levels of maximal respiration. Data are shown from at least three replicates as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparison test was used to calculate statistics: * p < 0.05, ** p < 0.01, **** p < 0.0001.