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. 2022 Oct 15;14(20):5060. doi: 10.3390/cancers14205060

Figure 4.

Figure 4

Hypoxia induces the expression of PHGDH in human colorectal cancers. (A) Correlation between PHGDH overexpression (p ≥ 1.5) and hypoxia by assessing the Buffa, Winter and Ragnum hypoxia scores accessed through cBioPortal for Cancer Genomics. (B) Representative Western blots of PHGDH, PSAT1 and PSPH with β-actin as loading control in HCT116 and DLD-1 cells under hypoxic conditions (0.1% O2). (C) Relative protein expression of PHGDH, PSAT1 and PSPH under hypoxic conditions (0.1% O2) normalized to the expression under normoxic conditions. (D) D-serine levels were determined upon treatment with NCT-503 (5 h) at indicated concentrations under hypoxic conditions (0.1% O2). Unpaired t-test (A) and ordinary one-way ANOVA with Dunnett’s multiple comparison test (D) were used to calculate statistics: * p < 0.05, *** p < 0.001, **** p < 0.0001.