Figure 5.

PHGDH inhibition mainly disrupts ROS homeostasis in human hypoxic colorectal cancer cells. HCT116 (top) and DLD-1 (bottom) cells were treated with NCT-503 for 5 h at indicated concentrations under hypoxic conditions (0.1% O₂). (A) aKG levels—normalized to control; (B) GSH levels—normalized to control; (C) NADPH levels—normalized to control; (D) ROS levels—normalized to control. Data are shown from at least four replicates as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparison test was used to calculate statistics: * p < 0.05, ** p < 0.01.