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. 2022 Sep 29;12(10):806. doi: 10.3390/bios12100806

Figure 5.

Figure 5

Epithelial sodium channel (ENaC) expression detected on platelet plasma membranes: (Aa) Representative bands corresponding to α-ENaC (55 kDa) and loading control GAPDH (37 kDa) detected by Western blotting assay from platelet lysates from individuals included in the study regardless of their health condition. (Ab) Levels of ENaC expression detected on platelet plasma membranes using the blotting assay. Band intensities are expressed as the relative number of pixels; **** p < 0.0001. (Ac) ROC curve. Black circles show the percentage of positive individuals for every cut-off value determined by Wb assay; red line represents the discrimination line, limiting the zone of no discrimination. (Ba) Representative fluorescence emission spectra of platelets samples λexc of 488 nm (495–570 nm). Samples corresponded to different individuals included in the study regardless of their health condition. (Bb) Levels of ENaC expression detected on platelet plasma membranes using the AuNPs-anti-ENaC assay. Band intensities are expressed as the relative number of pixels; **** p < 0.0001. (Bc) ROC curve. Black circles show the percentage of positive individuals for every cut-off value determined by AuNPs-anti-ENaC assay; red line represents the discrimination line, limiting the zone of no discrimination.