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. 2022 Oct 18;14(20):5090. doi: 10.3390/cancers14205090

Figure 4.

Figure 4

miR-214 over-expression induced the proinflammatory activation of RAW 264.7 macrophages. (A) Real-time qPCR of miR-214-3p/5p in RAW 264.7 macrophages where miR-214 was over-expressed compared to control cells (two-way ANOVA). (B) NO concentration in CM of control or miR-214-overexpressing RAW 264.7 macrophages (t-test). (C) Real-time qPCR of IL-1β, IL-6, and TNF-α mRNA in the control and miR-214-overexpressing RAW 264.7 macrophages (t-test). (D) ELISA of IL-1β, IL-6, and TNF-α released in CM of the control or miR-214-overexpressing RAW 264.7 macrophages (t-test). * p < 0.05, ** p < 0.01, and *** p < 0.001.