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. 2022 Oct 18;14(20):5090. doi: 10.3390/cancers14205090

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Vascular remodeling induced by the conditioned media of EV-treated macrophages. (A) Flow cytometric analysis of VE-cadherin protein expression in murine endothelial cells exposed to the CM of macrophages treated or not with melanoma-derived acid and control EV. (B) Permeability assay of murine endothelial cells exposed to the CM of macrophages treated or not with melanoma-derived acid and control EV. (C) Trans-endothelial migration of B16-CFSE melanoma cells through a monolayer of murine endothelial cells exposed to the CM of macrophages treated or not with melanoma-derived acid and control EV (one-way ANOVA; * p < 0.05, ** p < 0.01, and *** p < 0.001).