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. 2022 Oct 11;11(20):3186. doi: 10.3390/cells11203186

Figure 3.

Figure 3

AtRA and RARα promote macrophage cholesterol efflux. (A–C) Peritoneal macrophages were isolated from Rarαfl/fl mice or RarαMac-/- mice, and then treated with Ac-LDL (25 mg/mL) for 48 h. The cells were also treated with vehicle or AtRA (2 μM). Western blot assays were performed (A), and ABCA1 (B) or ABCG1 (C) protein levels were quantified (n = 3). (D,E) Peritoneal macrophages were isolated from Rarαfl/fl mice or RarαMac-/- mice, and then treated with vehicle (D) or Ac-LDL (25 mg/mL) (E) for 48 h. The cells were also treated with vehicle or AtRA (2 μM). Cholesterol efflux to HDL was performed (n = 6). In (B–E), ordinary two-way ANOVA with Tukey’s multiple comparisons test was used for statistical analysis. All the data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01.