Table 1.
Oligonucleotide primers sequences used to amplify the genes of interest. Sequences were optimised for use in real-time qPCR studies and SYBR green technology, with predicted amplicons < 165 bp.
| Gene | Forward Sequence 5′–3′ Reverse Sequence 3′–5′ |
Tm (°C) | Product Size | Accession No. |
|---|---|---|---|---|
| IL-1 β | GCTACCTGTGTCTTTCCCGT CATCTCGGAGCCTGTAGTGC |
59.68 60.25 |
164 | NM_008361.4 |
| Itgam | GAGCAGGGGTCATTCGCTAC GCTGGCTTAGATGCGATGGT |
60.53 60.53 |
94 | NM_001082960.1 |
| Il-6 | CCCCAATTTCCAATGCTCTCC CGCACTAGGTTTGCCGAGTA |
59.24 60.11 |
141 | NM_031168.2 |
| Cd68 | CTCCCACCACAAATGGCACT CTTGGACCTTGGACTAGGCG |
60.54 60.11 |
95 | NM_001291058.1 |
| Mhc-2a | CAAGCTGTCTTATCTCACCTTCA ATCTCAGGTTCCCAGTGTTTCA |
60.34 61.81 |
108 | NM_010378.3 |
| Mmp-9 | ATCATAGAGGAAGCCCATTACAG TTTGACGTCCAGAGAAGAAGAAA |
59.86 59.96 |
129 | NM_013599.4 |
| Nos2 | TACCAAAGTGACCTGAAAGAGG TCATCTTGTATTGTTGGGCTGA |
60.06 59.96 |
89 | NM_010927.4 |
| Il-10 | GCATGGCCCAGAAATCAAGG GAGAAATCGATGACAGCGCC |
59.54 59.42 |
91 | NM_010548.2 |
| Arg-1 | ACAAGACAGGGCTCCTTTCAG TTAAAGCCACTGCCGTGTTC |
59.93 59.05 |
105 | NM_007482.3 |
| Aif1 | ACGTTCAGCTACTCTGACTTTC GTTGGCCTCTTGTGTTCTTTG |
60.23 60.18 |
107 | NM_001361501.1 |
| Bdnf | CGAGTGGGTCACAGCGGCAG GCCCCTGCAGCCTTCCTTGG |
60.04 59.97 |
160 | NM_007540.4 |
| S18 | CCCTGAGAAGTTCCAGCACA GGTGAGGTCGATGTCTGCTT |
59.60 59.75 |
145 | NM_011296.2 |