Figure 1.

Analysis of miRNA expression in the knockout cells by Northern blotting. Total RNA (10µg/lane) from the BJAB-wild-type and SUDHL4-wild-type (“WT”) cells and the corresponding miR-142 knockout (“KO”) cells was separated on a 12% polyacrylamide gel, transferred to a nitrocellulose membrane and hybridized with ³²P-labelled probe for miR-142-3p (left upper panel) or miR-142-5p (right upper panel). Bound probe was detected using a PhosphoImager. The bound probes were removed and the membranes were re-hybridized either with a probe to detect miR-21 (left side of middle panel) or U6 RNA (right side of middle panel) as indicated. The bottom panel shows the Ethidiumbromide (“Etbr”)-stained gels as loading control.