Figure 7.

Viability of (a) normal human foetal osteoblasts (hFOB 1.19, ATCC CRL-11372) and (b) normal mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4, ATCC CRL-2593) after 24 and 48 h incubation with extracts obtained from Cur_WPI25_HAp and Cur_WPI35_HAp biomaterials. The extracts from biomaterials were prepared according to ISO 10993-12:2012 standard: Biological evaluation of medical devices—Part 12: Sample preparation and reference materials [33]. Control extracts were obtained by incubation of culture media in non-cytotoxic polystyrene culture plate. The biomaterials were incubated at 37 °C for 24 h. Cell viability was assessed using the MTT assay. The unpaired Student’s t-test was performed in order to determine statistical differences between samples, p < 0.05: * Significantly different results compared to control extracts.