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. 2022 Oct 16;11(20):3251. doi: 10.3390/cells11203251

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Levels of osteogenic markers: (a) collagen I (COLI), (b) bone alkaline phosphate (bALP), and (c) osteocalcin (OC) in normal human foetal osteoblasts (hFOB 1.19, ATCC CRL-11372) after 7, 14, and 21 days of cell culture on polystyrene (PS, control), Cur_WPI25_HAp, and Cur_WPI35_HAp biomaterials. Results were obtained using ELISA tests. The normal distribution of data was analyzed using a D’Agostino and Pearson omnibus normality test, then one-way ANOVA test followed by Tukey’s multiple comparison were performed in order to determine statistical differences between samples, p < 0.05: * Significantly different results between Cur_WPI25_HAp or Cur_WPI35_HAp biomaterials and polystyrene (PS) at the same time of experiment; ^$ significantly different results between Cur_WPI25_HAp and Cur_WPI35_HAp biomaterials; ^# significantly different results compared to data obtained after 7 days; ^% significantly different results compared to data obtained after 14 days.