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. 2022 Oct 14;11(20):3219. doi: 10.3390/cells11203219

Figure 9.

Figure 9

Human Panx1 conductance is activated by cholesterol depletion. (A,B) Electrophysiological recordings obtained from N2a cells expressing hPanx1 subjected to treatment with 5 mM MβCD (A,D) and 5 µM lovastatin (B,E). Note that N2a hPanx-1 transfected cells responded to cholesterol depletion with increased current amplitudes (red and green curves, in A and B, respectively) in response to 5.5 s-long voltage ramps from −60 to +80 mV compared to untreated cells, CTRL (black curves). Mean ± SEM values of the fold changes in peak conductance induced by 5 mM MβCD and 10 µM lovastatin obtained for N2a hPanx1 transfected cells are shown in parts (C,D), respectively. p values were obtained using Mann–Whitney tests. n = 10–11 cells. ** p < 0.01, *** p < 0.001. Traces in (C) and quantification in (F) correspond to recordings obtained from the Panx1null cells with and without MβCD treatment.