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. 2022 Oct 6;13(5):e02375-22. doi: 10.1128/mbio.02375-22

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Copyright © 2022 da Silva Barreira et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Purified MVs from L. casei BL23 are characterized for size and protein composition. (A) Cryo-SEM image of purified MVs. (A, inset) Zoomed-in view of the Cryo-SEM image. (B) Negative-staining TEM images of purified MVs. (C) The size distribution of purified MVs was obtained by analysis of the Cryo-SEM (830 vesicles) and the negative-staining TEM (533 vesicles) images. Vesicle sizes were obtained using the image analysis software Fiji (37). (D) Functional classification of L. casei BL23 MV proteins.