TABLE 2.
N-Succinylation of the tested antibiotics by recombinant CsbC protein
| Tested substrate | No. of: |
||
|---|---|---|---|
| Primary/secondary aliphatic amine groups | Aromatic amine groups | Succinyl groups added by CsbC | |
| Ampicillin | 1/0 | 0 | 1 |
| Colistin | 5/0 | 0 | 1–5 |
| Daptomycin | 1/0 | 1 | 1 |
| Desertomycin A | 1/0 | 0 | 1 |
| Ethionamide | 0/0 | 0 | 0 |
| Gentamicin | 3/2a | 0 | 1–3 (4 in traces) |
| Kanamycin | 4/0b | 0 | 1–4 |
| Tetracycline | 0/0c | 0 | 1 |
| Trimethoprim | 0/0 | 2 | 1 (traces) |
| Vancomycin | 1/1 | 0 | 1 (2 in traces) |
a
For gentamicin C1.
b
For kanamycins A and C.
c
One tertiary aliphatic amine that cannot be succinylated.