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. 2022 Oct 19;44(10):5048–5066. doi: 10.3390/cimb44100343

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of TSE on expression levels of p- JUN/ JUN, p-ERK1/2/ ERK1/2, p-p38/ p38, p-PI3K/ PI3K, p-mTOR/ mTOR, p-Akt (Ser⁴⁷³)/ t-Akt, and p-Akt (Thr³⁰⁸)/ t-Akt in SRA01/04 cells by Western blot. (A) representative image, and (B) quantification of the p- JUN/ JUN, p-ERK1/2/ ERK1/2, and p-p38/ p38 to β-actin. Protein was separated by 12% SDS-PAGE. (C) representative image, and (D) quantification of p-PI3K/ PI3K, p-mTOR/ mTOR, p-Akt (Ser⁴⁷³)/ t-Akt, and p-Akt (Thr³⁰⁸)/ t-Akt to β-actin. Protein was separated by 12% SDS-PAGE. (C) representative image, and (D) quantification of p-PI3K, p-mTOR, p-Akt (Ser⁴⁷³), and p-Akt (Thr³⁰⁸) to β-actin. ^# p < 0.05 or ^## p < 0.01 in comparison to control cells. * p < 0.05 or *** p < 0.001 in comparison to TGF-β2-induced control cells. Data are the means ±SE (n = 3).

Effects of TSE on expression levels of p- JUN/ JUN, p-ERK1/2/ ERK1/2, p-p38/ p38, p-PI3K/ PI3K, p-mTOR/ mTOR, p-Akt (Ser⁴⁷³)/ t-Akt, and p-Akt (Thr³⁰⁸)/ t-Akt in SRA01/04 cells by Western blot. (A) representative image, and (B) quantification of the p- JUN/ JUN, p-ERK1/2/ ERK1/2, and p-p38/ p38 to β-actin. Protein was separated by 12% SDS-PAGE. (C) representative image, and (D) quantification of p-PI3K/ PI3K, p-mTOR/ mTOR, p-Akt (Ser⁴⁷³)/ t-Akt, and p-Akt (Thr³⁰⁸)/ t-Akt to β-actin. Protein was separated by 12% SDS-PAGE. (C) representative image, and (D) quantification of p-PI3K, p-mTOR, p-Akt (Ser⁴⁷³), and p-Akt (Thr³⁰⁸) to β-actin. ^# p < 0.05 or ^## p < 0.01 in comparison to control cells. * p < 0.05 or *** p < 0.001 in comparison to TGF-β2-induced control cells. Data are the means ±SE (n = 3).