TABLE 1.
Antibodies
| Primary antibody | Antibody species | Blocking buffera | Dilution | Catalog no. |
|---|---|---|---|---|
| Phospho-IRE1α | Rabbit | 5% BSA/TBST | 1:1,000 | Abcam EPR5253 Invitrogen PA585738 |
| IRE1α (14C10) | Rabbit | 5% Milk/TBST | 1:1,000 | Cell Signaling Technology 3294S |
| XBP1s | Mouse | 5% Milk/TBST | 1:1,000 | BioLegend 9D11A43 |
| GAPDH (14C10) | Rabbit | 5% Milk/TBST | 1:2,000 | Cell Signaling Technology 2118S |
| SARS-CoV-2 N | Rabbit | 5% Milk/TBST | 1:2,000 | Gentex GTX135357 |
| MERS-CoV N | Mouse | 5% Milk/TBST | 1:2,000 | Sino Biological40068-MM10 |
| OC43 N | Rabbit | 5% Milk/TBST | 1:2,000 | Sino Biological 40643-T62 |
a
TBST, Tris-buffered saline with Tween 20.