TABLE 3.
Sequences of PCR primers available for diagnosis of Whipple's disease
| Primer(s) | Sequence | Reference |
|---|---|---|
| W3FE | 5′-GGA ATT CCA GAG ATA CGC CCC CCG CAA-3′ | 50 |
| W2RB | 5′-CGG GAT CCC ATT CGC TCC ACC TTG CGA-3′ | 50 |
| W4RB | 5′-CGG GAT CCT GTG AGT CCC CGC CAT TAC GC-3′ | 51 |
| TW-1 and whip1 | 5′-AGA GAT ACG CCC CCC GCA A-3′ | 21, 61 |
| TW-3 | 5′-TCC TGT GAG TCC CCG CCA TTA GGC-3′ | 21 |
| W3AF | 5′-TAC CGG AAA GGC GTA GAG ATA CGC C-3′ | 47 |
| W4AR | 5′-CAG TCT CCT GTG AGT CCC CGC CAT T-3′ | 47 |
| Whip2 | 5′-ATT CGC TCC ACC TTG CGA-3′ | 61 |
| W185 | 5′-CGA CCC ATG AGG GCA TCC TC-3′ | 12 |
| RW830 | 5′-GCG GTG GAA CCA CCC CCA CG-3′ | 12 |
| tw1662f | 5′-ACT ATT GGG TTT TGA GAG GC-3′ | 28 |
| tw1662r | 5′-GCC TCT CAA AAC CCA ATA GT-3′ | 28 |
| tw1857r1 | 5′-TCC CGA GCC TTA TCC GAG A-3′ | 28 |
| tw1857r2 | 5′-TCC CGA GGC TTA TCG CAC A-3′ | 28 |
| tws1,f | 5′-ATC GCA AGG TGG AGC GAA TCT-3′ | 27, 28 |
| tws2,r | 5′-CGC ATT CTG GCG CCC CAC-3′ | 27, 28 |
| tws3,f | 5′-CCG GTG ACT TAA CCT TTT TGG AGA-3′ | 27, 28 |
| tws4,r | 5′-TCC CGA GGC TTA TCG CAG ATT G-3′ | 27, 28 |
| twsA1/f | 5′-AAG TGA TAC CGC CAT AGT GCA CTG T-3′ | 27 |
| twsA2/f | 5′-AAG TGA TAC CGC CAT AGT GCA CTG C-3′ | 27 |
| twsB1/r | 5′-CTC CCG TGA GCT TGT GCC CAA AAC-3′ | 27 |
| twsB2/r | 5′-CTC CCG TGA GCT TGT GCC CAA AC-3′ | 27 |
| twsC1/r | 5′-AAT AGT GCA CAC AAG TGC ATA AGC A-3′ | 27 |
| twC2/r | 5′-AAT AGT GCA CAC AAG CGC ATA AGC A-3′ | 27 |
| HGC-23InsF | 5′-CGT AGT CGA TGG ACA ACG-3′ | 26 |
| TW-23InsR1 | 5′-TAG AAC CTT GTG TCG ATG C-3′ | 26 |
| TW-23InsF | 5′-GGT TGA TAT TCC CGT ACC GGC AAA G-3′ | 26 |
| TW-23InsR2 | 5′-GCA TAG GAT CAC CAA TTT CGC GCC-3′ | 26 |
| whipp-f | 5′-GCC TGC GCC TCG ATC TCT GC-3′ | 42 |
| whipp-frw1 | 5′-TGA CGG GAC CAC AAC ATC TG-3′ | 42 |
| whipp-frw2 | 5′-CGC GAA AGA GGT TGA GAC TG-3′ | 42 |
| whipp-rev | 5′-ACA TCT TCA GCA ATG ATA AGA AGT T-3′ | 42 |
| 16Spro1 | 5′-TTG AGA ACT CAA BAG YGT G-3′ | 42 |
| tw318r | 5′-CGA AGT TAT CCC AAA GTT AG-3′ | 42 |
| tw1581f | 5′-GTG ACT TAA CCT TTT TGG AGA-3′ | 33 |
| tw2015r | 5′-GCA TCC ACC ATT TGC TCT TAA A-3′ | 33 |
| tw1974f | 5′-GTA TTT GTG ATT CAA GCT AC-3′ | 33 |
| ms37ar | 5′-CTG CTT CTA AGC CAA CAT CCT-3′ | 33 |
| tw3002f | 5′-TGC CGG TAA GTT AGA GCG CA-3′ | 33 |
| ms38a | 5′-GAC AAG GAA TTT CGC TAC CTT A-3′ | 33 |
| tw3887f | 5′-GCC TGA GGC GTG ACG AG-3′ | 33 |
| co5189r | 5′-GCT TCC GGG TTC GGA ATG-3′ | 33 |
| tw5104f | 5′-CTT GAT GTG CGG CCC TTT GC-3′ | 33 |
| tw5745r | 5′-AAG ATC CCA CTG CAC TGA CAT CG-3′ | 33 |
| tw5068f | 5′-ATT GCT TGA AAC ACA TTT TG-3′ | 33 |
| NheI-RSO | 5′-AAT ACG ACT CAC TAT AGC N10GC TAG C-3′ | 33 |