FIG 1.

Eukaryotic virome profile in Aedes aegypti and Culex quinquefasciatus. (a) Abundance of eukaryotic viral species in Aedes aegypti. The plaque assay results are indicated by “+” for positive results and “–” for negative results in the gray bars. The dot plots show the total ZIKV genome copies in Aedes aegypti heads and bodies determined by qRT-PCR. The heatmap shows the read counts on log₁₀ scale. The viral species names shown in the heatmap are from the taxonomic annotation by DIAMOND and KronaTools. The heatmap only shows the viral species and samples that had more than 500 eukaryotic viral reads (21 viral species and two samples were removed). (b) Alpha diversity of eukaryotic viral species in Aedes aegypti at 7 and 21 dpe. Pairwise Wilcoxon test: P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), P < 0.0001 (****). P values were corrected for multiple comparisons using the Benjamini-Hochberg method. (c and d) PCoA of eukaryotic viral species in Aedes aegypti based on Bray-Curtis dissimilarity. The PERMANOVA test was performed on food sources. The “+” or “–” in the bracket after ZIKV indicates the positive or negative result of plaque assay (head), qRT-PCR detection of ZIKV in head and body, respectively. (e) Abundance of eukaryotic viral species in Culex quinquefasciatus. The plaque assay results are indicated by “+” for positive results and “–” for negative results in the gray bars. The dot plots show the total WNV genome copies in Culex quinquefasciatus heads and bodies determined by qRT-PCR. The heatmap shows the reads counts on log₁₀ scale. The viral species names shown in the heatmap are from the taxonomic annotation by DIAMOND and KronaTools. Three samples with zero eukaryotic viral reads and 16 species containing less than 500 reads were deleted from the heatmap. Note: No qRT-PCR was performed for WNV on the heads of Culex quinquefasciatus mosquitoes, due to insufficient sample.