FIG 4.

Disruption of codY or lpdA affects membrane fatty acid composition. (A) Fatty acid composition was measured in exponential-phase cells using gas chromatographic analysis of fatty acid methyl esters (GC-FAME) (n = 3). (B) Membrane polarization was measured in exponential-phase cells. Membrane polarization is inversely correlated with membrane fluidity (n = 4). (C) GC-FAME for strains grown to exponential phase supplemented with 0.5 mM sBCFAs. Because samples were prepared and analyzed separately, comparisons between panels A and C are not made (n = 3). For all panels, bars represent mean ± standard deviation. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant compared to wild type under that condition, by either ANOVA with Tukey’s post hoc test (A) or ANOVA followed by Student’s t tests with Bonferroni correction (B and C).