Figure 4.

Deducing the type of inhibition of compound 1 against CA-II. (A) The first graph is drawn (Lineweaver–Burk plot) between the reciprocal of reaction rate (velocity) vs. reciprocal of substrate (p-Nitro phenyl acetate) in the presence of highest dose 25 µM (○), second highest dose, and so on: 20 µM (●), 15 µM (□), 10 µM (■), 5 µM (∆), and negative control (▲) of compound 1. (B) The second graph is (Lineweaver–Burk plot) between the gradient of each line vs. different concentrations of inhibitor “compound 1”. (C) The third graph “Dixon plot” is drawn between reciprocal of reaction rate (velocities) vs. different concentrations of inhibitor “compound 1”.