FIG. 6.

Effect of 1.5% H₂O₂, 0.016% Sag 471, 0.003% NaN₃, and/or 5mM EDTA on whole blood. (A) Solution containing all four components. (B) Solution containing H₂O₂, NaN₃, and EDTA. (C) Solution containing H₂O₂, Sag 471, and EDTA. Lanes: a, 10 μl of whole blood; b, 20 μl of whole blood; c, 30 μl of whole blood; d, 40 μl of whole blood. Sag 471 somewhat reduced the decoloring activities of H₂O₂ (C); however, this activity was recovered by the addition of NaN₃ (A). NaN₃ helped H₂O₂ to effectively decolor RBCs yet restrained the mixture from developing through the NC membrane (B).