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. 2022 Oct 20;14(20):5148. doi: 10.3390/cancers14205148

Figure 4.

Figure 4

Knockdown of METTL3 influences androgen-induced transcriptional programmes in PCa cell lines. PCa cells treated with −/+ 1nM R1881 and −/+ siMETTL3 were analysed by RNA-seq and the DEGs determined. METTL3 knockdown in LNCaP:C4-2 (A) and 22Rv1 (C) −/+ R1881 was confirmed by qRT-PCR and Western blotting. Heatmap of AR-regulated genes LNCaP:C4-2 (B) and 22Rv1 (D) siSCR and siMETTL3 treated with Veh or R1881. The androgen-induced differential gene expression and top significantly enriched KEGG pathways in siSCR Veh vs. R1881 and siMETTL3 Veh vs. R1881 in LNCaP:C4-2 (E,G) and 22Rv1 (F,H) is shown. Genes with significantly higher expression with R1881 treatment are indicated in red and genes significantly lower with R1881 treatment are indicated in green. Non-significantly DEGs are plotted in black. Common and uniquely regulated genes in siSCR vs. siMETTL3 and enriched KEGG pathways with METTL3 dependant R1881 regulated genes in LNCaP:C4-2 (I) and 22Rv1 (J) are shown. Significant gene expression: FC ±1.5 and FDR < 0.05. **** p ≤ 0.0001. Molecular weights for proteins are indicated in the full uncropped annotated Western blot images (Figures S8 and S9).