Figure 5.

Knockdown of METTL3 influences androgen-induced splicing in PCa cell lines. PCa cells treated with −/+ 1nM R1881 and −/+ siMETTL3 were analysed by RNA-seq and the DSGs determined. The significant androgen-regulated differential splicing events, genes, and top significantly enriched KEGG pathways in siSCR Veh vs. R1881 and siMETTL3 Veh vs. R1881 in LNCaP:C4-2 (A–D) and 22Rv1 (E–H) are shown. Significant splicing events: dPSI ≥ 5% and FDR < 0.05. Veh = vehicle, FC = fold-change, FDR = false discovery rate. SE = skipped exon, MXE = mutually exclusive exon, RI = retained intron, A5SS = alternative 5′ splice site, A3SS = alternative 3′ splice site.