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. 2022 Oct 11;11(20):3182. doi: 10.3390/cells11203182

Figure 2.

Figure 2

RB1 and HK1 complementation in Rb null cells reveals distinct energy profiles. (A) Western blot showing the protein expression of phospho-Rb, Rb, E2F2, and HK1 in RB1 null and RB1-overexpressed (OE) WERI-Rb1 cells modulated with E2F2 OE and HK1 OE and knockdown constructs. Energy phenotype profile of (B) vector control and RB1-overexpressed WERI-Rb1 cells, (C) HK1 OE cells vs. RB1 OE+ HK1 OE WERI-Rb1 cells, and (D) HK1 knockdown vs. RB1 OE+ HK1 KD cells. (E) Metabolic potential of modulated cells under stressed phenotype. Open squares represent baseline activity, and closed squares represent stress-induced activity. Values represent three independent experiments mean with data points ± s.d. Kruskal–Wallis with Dunn’s multiple comparisons test (for >2 groups) were used for statistical analysis. ** p < 0.01, *** p < 0.001. OE = overexpression; KD = knockdown.