Figure 2.
(A–F). VEGFA isoforms and Rock1 in diabetic rat retina before and after NGF ocular treatment. Levels of VEGFA isoforms 121, 165, 189 (30 kDa, 46 kDa and 50 kDa, respectively) and Rock1 were affected by diabetes (STZ) and NGF eye drops administration (STZ+NGF) as shown by Western Blots (A,E) and densitometric analyses (B–D,F). β-Actin expression was used to normalize sample variability; a.u. indicates arbitrary units. The results are expressed as mean ±SD. Statistical significance is indicated with * p < 0.05, *** p < 0.001. “a” vs. CTRL, “b” vs. STZ. CTRL, healthy rats; STZ, streptozotocin; STZ+NGF, streptozotocin+nerve growth factor eye drops.