Figure 3.

(A–D). VEGFR2 and its phosphorylated status (pVEGFR2) levels in diabetic rat retina, before and after NGF ocular treatment. VEGFR2 and pVEGFR2 levels altered in STZ and STZ+NGF groups, as showed by Western Blot (A) and densitometric analyses (B–D). β-Actin expression was used to normalize sample variability, a.u. indicates arbitrary units. The results are expressed as mean ±SD. Statistical significance is indicated with * p < 0.05, ** p < 0.01, *** p < 0.001. “a” vs. CTRL group; “b” vs. STZ group. CTRL, healthy rats; STZ, streptozotocin; STZ+NGF, streptozotocin+nerve growth factor eye drops.