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Vinculin expression in CRC cell lines HCT15 (top), HCT116 (middle), and SW620 (lower images) immunostained for DAPI to illustrate the nuclei (blue), vinculin (cyan), and phalloidin for F-actin filaments (red). The bottom Z-stack micrograph is shown, and a Z-maximum projection showing the overall stains is also represented (right panels). Scale bars: 10 µm.
