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. 2022 Oct 18;11(20):3278. doi: 10.3390/cells11203278

Figure 1.

Figure 1

Representative images of ECs under different experimental settings. Tube assay on MatrigelTM (19 h). HUVECs were seeded on MatrigelTM at a density of 5 × 104 cells/cm2 and cultured for 19 h in EGM-2 (Lonza). Co-culture model. HUVECs were preliminarily labeled with vital fluorescent dye CMFDA (green). The mixtures of HUVEC:MSC at a ratio of 1:3 and a density of 6 × 104 cells/cm2 were seeded onto a Petri dish without matrix and co-cultured in EGM-2 (Lonza) for 48 h. Proliferating/motile culture of HUVECs: sparsely populated monoculture (3.6 × 104 cells/cm2) in EGM-2 (Lonza) for 19 h. Static monolayer of densely populated HUVECs (7 × 104 cells/cm2) for 19 h in EGM-2 (Lonza). Images were taken using the Axiovert 200 M Microscope (Zeiss, Oberkochen, Germany): 10× amplification (a), 68× amplification (b) of selected areas, where white arrows indicate tip cells among others. (c) A schematic representation of the formed cellular structures.