Figure 2.

(a) Venn diagram summarizes the number of significantly changed genes (p < 0.05) that are differentially upregulated or downregulated between experimental groups compared to the HUVEC static monolayer. ECs from direct co-culture were separated using anti CD31 magnetic beads. Total RNA was isolated and specific mRNA levels were quantified by qRT PCR as described in “Methods”. (b) Hierarchical clustering dendrograms of differentially expressed mRNA expression patterns in HUVECs on Matrigel^TM, in co-culture with MSCs, and proliferating/motile HUVECs analyzed using online software Heatmapper [8]. The data are presented in three repeats. Clustering Method: Average linkage. Distance Measurement Method: Pearson. (c) Hierarchical clustering dendrograms of differentially expressed mRNA expression patterns in HUVECs on Matrigel^TM and proliferating/motile HUVECs analyzed using the online software Heatmapper [8]. The data are presented in three repeats. Clustering Method: Average linkage. Distance Measurement Method: Pearson.