FIG 5.

Effect of full-length mRNA molecule on RNA editing of FG3G34330. (A) and (C) Sequencing traces for flanking sequences of each editing site of FG3G34330 amplified from RNA isolated from perithecia of wild type (WT), transformants ectopically expressing the full-length transcript (pWT) or transcript without 3′-untranslated region (UTR) (pΔ3’UTR) or with only the 5′-UTR and the first 137 bp coding sequences (p5’part), and in situ 3′ UTR deletion mutant (Δ3’UTR). Red lines mark the editing sites with mixed peaks of A and G although only the dominating peak is shown in the sequence on the top. (B) and (D) Means and standard deviations of the editing levels were estimated from 3 biological replicates (n = 3). Significant differences for pairwise comparison are based on t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant). (E) RNA secondary structure plots of the full-length transcript (−330 to 1278 bp relative to translational initiation), transcript without 3′-UTR (−333 to 1047 bp), and 5′ partial transcript (−330 to 137 bp) of FG3G34330. Colors represent the base-pair probabilities. The 4 A-to-I editing sites are indicated.