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. 2022 Oct 11;11(20):3188. doi: 10.3390/cells11203188

Figure 1.

Figure 1

The effect of PFN1 on the differentiation of bovine skeletal muscle satellite cells: (A) qRT-PCR detection of mRNA expression level of differentiation marker genes in si-PFN1 or si-NC transfected DM2 myoblasts; (B) qRT-PCR detection of the mRNA expression level of differentiation marker genes in pcDNA-PFN1 or pcDNA3.1(+) transfected DM2 myoblasts; (C) Western blot and quantitative analysis of differentiation marker protein levels in si-PFN1 or si-NC transfected DM2 myoblasts; (D) Western blot and quantitative analysis of differentiation marker protein levels in pcDNA-PFN1 or pcDNA3.1(+) transfected DM2 myoblasts; (E) Myotube formation status in si-PFN1 or si-NC transfected DM2 myoblasts; (F) Myotube formation status during differentiation in pcDNA-PFN1 or pcDNA3.1(+) transfected DM2 myoblasts. si-NC stands for negative control, and pcDNA3.1(+) stands for empty vector control negative control. * p < 0.05, ** p < 0.01, and *** p < 0.001.