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. 2022 Oct 11;11(20):3188. doi: 10.3390/cells11203188

Figure 4.

Figure 4

PFN1-Cdc42 negatively regulates bovine skeletal muscle via PAK/JNK signaling pathway: (A) Western blot testing and quantitative analysis of the effect of Cdc42 on PAK phosphorylation in si-Cdc42 or si-NC transfected DM2 myoblasts; (B) Western blot testing and quantitative analysis of the effect of Cdc42 on PAK phosphorylation in pcDNA-Cdc42 or pcDNA3.1(+) transfected DM2 myoblasts; (C) changes analysis of differentiation marker proteins of the DM2 bovine myoblasts with P-PAK inhibitor by Western blot; (D) protein level analysis of P-JNK after P-PAK inhibition in DM2 bovine myoblasts by Western blot; (E) Western blot detecting and quantitative analysis of the effect of PFN1 on the JNK signal pathway in si-PFN1 or si-NC transfected DM2 myoblasts; (F) Western blot detecting and quantitative analysis of the effect of PFN1 on the JNK signal pathway in pcDNA-PFN1 or pcDNA3.1(+) transfected DM2 myoblasts; (G) Western blot detecting and quantitative analysis of the effect of Cdc42 on the JNK signal pathway in si-Cdc42 or si-NC transfected DM2 myoblasts; (H) the effect analysis of Cdc42 on the JNK signal pathway in pcDNA-Cdc42 or pcDNA3.1(+) transfected DM2 myoblasts; (I) change analysis of differentiation marker proteins of the DM2 bovine myoblasts with JNK inhibitor. si-NC stands for negative control, and pcDNA3.1(+) stands for empty vector control negative control. N.S.: non significance, * p < 0.05, ** p < 0.01.