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. 2022 Oct 17;11(20):3253. doi: 10.3390/cells11203253

Figure 3.

Figure 3

Agomir-let-7i suppressed HSI-induced neuroinflammation. (A) qPCR analysis showing the relative mRNA levels of the pro-inflammatory mediators IL-6, IL-1β, and TNF-α in the hippocampi of agomir-let-7i-treated and scramble control-treated mice at day 3 after HSI. GAPDH was used as the internal control. (B) Representative images of Iba1+ microglia in the impacted and peri-lesional areas at day 3 after HSI. Scale bar, 50 µm. (C) The binary transformation of the microglia morphology. (D) Quantification of the soma size and branch numbers of microglia at the lesion site. (E) Representative images of GFAP and Iba1 immunohistochemistry staining of the hippocampi at day 7 after HSI. Scale bar, 100 µm. (F) Quantification of the glial scar size in the hippocampi at day 7 after HSI. The dotted lines indicate the boundary of glial scars. Data are presented as mean ± SEM. n = 3 mice per group. * p < 0.05, ** p < 0.01, *** p < 0.001.