FIG. 1.

Nitrocellulose dot blot of anti-SU MAbs with native or denatured and reduced SU. The indicated concentrations of affinity-purified native SU (N) or SU denatured and reduced in SDS with 2-mercaptoethanol (D) were immobilized on nitrocellulose membranes and reacted with murine anti-SU MAb 74A (25 μg/ml), 16A (3 μg/ml), or 29A (50 μg/ml), irrelevant isotype control MAb 79/17 (50 μg/ml), or caprine MAb F7-299 (50 μg/ml). Negative control serum from uninfected goat 8505 (G05) and positive control serum from CAEV SU-immunized goat 9308 (G08) were used at dilutions of 1:1,000.