FIG. 5.

Western blot of anti-CAEV-63 SU MAbs with CAEV-Co SU. CAEV-63 (A) and CAEV-Co (B) were recovered from the medium of infected GSM cells and disrupted in 2% SDS without DTT (lanes designated with a minus sign) or with 100 mM DTT (lanes designated with a plus sign). Following addition of 100 mM IA, lysates of CAEV-63 (7 μg) and CAEV-Co (5 μg) were analyzed by Western blotting with anti-CAEV-63 SU or an irrelevant isotype control MAb. Negative control serum from goat 8505 (G05) and positive control serum, from goat 9808 (G08) were used at dilutions of 1:1,000.