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ELISA reactivity of HRP-conjugated MAb 74A with CAEV-63 SU captured by MAb F7-299. Affinity-purified SU (0.06 μg) or 100 μl of CM from CAEV-63-infected GSM cells was added to Immulon 2 wells coated with 100 ng of MAb F7-299. The wells were reacted with 0.06 μg of HRP-conjugated MAb 74A with or without preincubation with 75 μl of undiluted goat sera. (A) Purified SU captured with MAb F7-299. (B) Soluble SU from CM captured with MAb F7-299.
