FIG 1.

LEN binding to cross-linked HIV-1 CA hexamers. (A to D). Representative binding sensorgrams illustrating interactions between LEN with wild-type (WT) and mutant cross-linked HIV-1 CA hexamers evaluated by SPR detection. The binding data (black lines) were fit (orange lines) to a simple kinetic model with a mass transport added as needed. The mean ± standard deviation (SD) equilibrium dissociation constant (K_D), association rate constant (k_on), and dissociation rate constant (k_off) values were determined from three independent experiments with comparable results. The LEN concentrations are 0.156, 0.312, 0.625, 1.25, and 2.5 μM in (A); 0.078, 0.156, 0.312, 0.625, and 1.25 μM in (B); 0.156, 0.312, 0.625, and 1.25 μM in (C); and 0.078, 0.156, 0.312, 0.625, and 2.5 μM in (D). (E) Fold change of the K_D, k_on, and k_off values for the mutant CA variants compared to WT CA. P values indicate differences compared to CA(WT) hexamers.