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The algorithm for result interpretation of the RIF-RDp assay. H526D and D516V mutations can be detected in the HEX channel and Cy5 channel, respectively, and identified as RR-TB. Other mutations were differentiated based on the ∆Tm cutoff value. If the ∆Tm cutoff value is ≥0.245 °C, it was interpreted as rifampicin-resistant tuberculosis (RR-TB) and if it is <0.245 °C, it was identified as rifampicin-susceptible tuberculosis (RS-TB).
