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. 2022 Sep 24;12(10):2307. doi: 10.3390/diagnostics12102307

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Validation of the RIF-RDp assay for detection of H526D and D516V mutant Mtb strains using 14 rifampicin-resistant M. tuberculosis (RR-Mtb) strains (7, 3, 3, and 1 strain of Class-I, Class-II, Class-III (H526D), and Class-IV SNP (D516V)), 20 rifampicin-susceptible M. tuberculosis (RS-Mtb) strains, H526D and D516V mutant controls, and M. tuberculosis H37Rv reference strain. The fluorescence signal detected in the HEX channel (a,b), or Cy5 channel (c,d) illustrates the detection of H526D or D516V mutant Mtb strains, respectively.