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LOD determination of the RIF-RDp assay for detection of (a) rpoB gene, (b) H526D mutation, and (c) D516V mutation. The duplicate DNA samples of M. tuberculosis H37Rv (H37Rv), and the H526D and D516V mutant controls using 2 × 106 to 2 × 101 genome copies (gc) of Mtb H37Rv and negative control (sterile distilled water).
