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The performance of the RIF-RDp assay in the detection of rifampicin-resistant M. tuberculosis (RR-Mtb). The normalized melting peaks (a), and normalized melting curves (b) clearly distinguished RR-Mtb from rifampicin-susceptible M. tuberculosis (RS-Mtb) isolates based on HRM analysis. RR-Mtb strains harboring H526D or D516V mutation were detected in the HEX (c) or Cy5 channels (d), respectively.
